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PureCube Nanodisc MSP（12-14nm）series
PureCube Nanodisc MSP（7-8nm）系列包含以下产品：MSP1E3D1, lyophilized protein。
This product is available with different species as its background. This is important for e.g. for secondary antibodies of a western blot.
FEATURE |
|
Available origins | Human, mouse |
Purity | > 90% (Determined by SDS-PAGE) |
Number of amino acids | · 256 (Human origin) · 254 (Mouse origin) |
Molecular Mass | · 29,982 Da (Human origin) · 29,786 (Mouse origin) |
Extinction coefficient (in water) ε280 | · 26,930 M-1cm-1 (Human origin) · 29,450 M-1cm-1 (Mouse origin) |
Buffer | 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA |
Sequence (Human origin), for the other origins please send request | G STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ |
Affinity tag | none |
Shipping Temperature | Room Temperature |
Storage of lyophilized protein | -20°C for several months |
Storage of reconstituted protein | 2-8°C for several days |
Helpful literature references | 1. Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44 2. Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563 3. Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925 |
Order number | Products | Shipment Temperature | Storage Temperature |
26162 | MSP1E3D1, lyophilized protein (2 mg) | Ambient temperature | -20°C |
26166 | MSP1E3D1, lyophilized protein (10 mg) | Ambient temperature | -20°C |
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