FEATURE

MSP1D1-His, lyophilized protein

Nanodisc Assembly Kit MSP1D1-His DMPC

Available origins

Human, rat, mouse, alpaca

Purity

> 90% (Determined by SDS-PAGE)

Number of amino acids

· 217 (Human origin)

· 214 (Mouse origin)

· 210 (Rat origin)

· 232 (Alpaca origin)

217

Molecular Mass

· 25,309 Da (Human origin)

· 24,928 Da (Mouse origin)

· 24,614 Da (Rat origin)

· 26,743 Da (Alpaca origin)

25,309 Da

Extinction coefficient (in water) ε280

· 25,440 M^-1cm^-1 (Human origin)

· 22,460 M^-1cm^-1 (Mouse origin)

· 33,460 M^-1cm^-1 (Rat origin)

· 41,940 M^-1cm^-1 (Alpaca origin)

25,440 M^-1cm^-1

Buffer

20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Sequence (Human origin), for the other origins please send request

MGSSHHHHHH ENLYFQ GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ

Affinity tag

N-Terminal His-tag

Shipping Temperature

Room Temperature

Storage of lyophilized protein

-20°C for several months

Storage of reconstituted protein

2-8°C for several days

Helpful literature references

1. Bayburt, T.H. et al. Reconstitution and imaging of a membrane protein in a nanometer-size phospholipid bilayer. J. Struct. Biol. (1998), 123(1):37-44

2. Civjan, N.R. et al. Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers. BioTechniques (2003) 35:556-563

3. Hagn, F. et al. Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins. J.Am.Chem. Soc. (2013), 135:1919-1925

4. Proverbio D., et al. Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artifical membrane environments. Biochim.Biophys. Acta (2013), 1828(9):2182-92

5. Roos, C., et al. 2014, High-level Cell-free production of membrane proteins with Nanodiscs. In: Alexandrov, K., and Johnston W.A. (eds) Cell-free protein synthesis: Methods and Protocols. Methods in Molecular Biology, vol. 1118, Springer Science+Business Media.