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PureCube DIBMA series（1）
DIMBMA 10,HEPES、DIBMA 10,TRIS、DIBMA 12,HEPES、DIBMA 12,TRIS
For the longest time, the science behind membrane proteins relied on detergents for both solubilization and stabilization. However, detergents such as DDM or LMNG come with their set of problems. A time-consuming screening process for the correct detergent and the constant need to add it to all buffers can be avoided. But this applies to all synthetic nanodiscs.
Figure 1 shows the amount of membrane protein of interest that was stabilized by a DIBMALP in comparison to a construct using the detergent DDM. As it can clearly be seen DDM has way fewer specific bands at the desired kDA values of around 40-60 kDa.
An alternative to traditional DIBMAs and their sensitivity to the presence of ions inside a buffer, are our modified DIBMAs with added Glucosamine or amino-functionalized diol. As figure 2 indicates, the increased tolerance of Ca2+ is necessary. Normally DIBMA starts to precipitate in Ca2+ holding buffers at concentrations of around 25 mM. Using DIBMA-Glycerol this tolerance increases to 50 mM. There is no precipitate visible at the bottom of the tube. In comparison, normal DIBMA shows a visible precipitate at 25 mM. In terms of Mg2+ tolerance traditional DIBMA and the Glycerol-DIBMA both show a high tolerance above 50 mM.
Fig. 2: DIBMA precipitation in relation to the ion concentration. The shown concentrations start at 5 mM and are increasing in 5 mM steps up to 50 mM.
FEATURES | |
Usage | Protein solubilization |
Formula Weight | 12,000 g/mol |
pH | 7.5 in buffer |
dn/dc | 1.35 M-1 |
Solubility | > 10 % in H2O |
Absorbance at 280 nm | < 0.3 (1 % solution) |
Mg2+ Tolerance | Dependend on DIBMA product |
Ca2+ Tolerance | Dependend on DIBMA product |
Order number | Products | Shipment Temperature | Storage Temperature |
18001 | PureCube DIBMA 10, Screening Kit, 10x50 mg, HEPES | Ambient temperature | -20°C |
18002 | PureCube DIBMA 10, 1g, HEPES | Ambient temperature | -20°C |
18021 | PureCube DIBMA 10, 10g, HEPES | Ambient temperature | -20°C |
18022 | PureCube DIBMA 10, 5x10g, HEPES | Ambient temperature | -20°C |
18004 | PureCube DIBMA 10, Screening Kit, 10x50 mg, TRIS | Ambient temperature | -20°C |
18005 | PureCube DIBMA 10, 1g, TRIS | Ambient temperature | -20°C |
18026 | PureCube DIBMA 10, 10g, TRIS | Ambient temperature | -20°C |
18027 | PureCube DIBMA 10, 5x10g, TRIS | Ambient temperature | -20°C |
18011 | PureCube DIBMA 12, Screening Kit, 10x50 mg, HEPES | Ambient temperature | -20°C |
18012 | PureCube DIBMA 12, 1g, HEPES | Ambient temperature | -20°C |
18031 | PureCube DIBMA 12, 10g, HEPES | Ambient temperature | -20°C |
18032 | PureCube DIBMA 12, 5x10g, HEPES | Ambient temperature | -20°C |
18014 | PureCube DIBMA 12, Screening Kit, 10x50 mg, TRIS | Ambient temperature | -20°C |
18015 | PureCube DIBMA 12, 1g, TRIS | Ambient temperature | -20°C |
18036 | PureCube DIBMA 12, 10g, TRIS | Ambient temperature | -20°C |
18037 | PureCube DIBMA 12, 5x10g, TRIS | Ambient temperature | -20°C |
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Hernandez L.M.R. & Levental I.
1.Biophysical Journal，Available online 14 January 2023.
Zhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C.,Smith Ill A.B.S, Sodroski J.G.
bioRxiv.
Voskoboynikova, N.; Orekhov, P.; Bozdaganyan, M.; Kodde, F.; Rademacher, M.; Schowe, M.; Budke-Gieseking, A.; Brickwedde, B.; Psathaki, O.-E.; Mulkidjanian, A.Y.; Cosentino, K.; Shaitan, K.V.; Steinhoff, H.-J.
MDPI.
Voskoboynikova, N.; Margheretis E.G., Kodde F., Redemacher M., Schowe M., Budke-Gieseking A., Psathaki O-E-, Steinhoff H-J., Cosentino K.
sciencedirect.
Brown C.J. , Trieber C., Overdiun M.
sciencedirect.
Overduin M., Trieber C., Prosser R.S., Picard L-P., Sheff J.G.
membranes.