Home
PureCube Glyco-DIBMA
Glyco-DIBMA is a modified version of DIBMA with an added sugar side-chain to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins.
After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose.
DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.
Figure 1 shows the amount of membrane protein of interest that was stabilized by a DIBMALP in comparison to a construct using the detergent DDM. As it can clearly be seen DDM has way fewer specific bands at the desired kDA values of around 40-60 kDa.
An alternative to traditional DIBMAs and their sensitivity to the presence of ions inside a buffer, are our modified DIBMAs with added Glucosamine or amino-functionalized diol. As figure 2 indicates, the increased tolerance of Ca2+ is necessary. Normally DIBMA starts to precipitate in Ca2+ holding buffers at concentrations of around 25 mM. Using DIBMA-Glycerol this tolerance increases to 50 mM. There is no precipitate visible at the bottom of the tube. In comparison, normal DIBMA shows a visible precipitate at 25 mM. In terms of Mg2+ tolerance traditional DIBMA and the Glycerol-DIBMA both show a high tolerance above 50 mM.
Fig. 2: DIBMA precipitation in relation to the ion concentration. The shown concentrations start at 5 mM and are increasing in 5 mM steps up to 50 mM.
FEATURES | |
State | Lyophilized Powder, |
Molecular weight | 15701 g/Mol |
pH after solving | 7.5 |
Solubility | >10% |
Divalent cationic tolerance | < 4 mM |
Adsorbance (280 nm, 1% solution) | > 0.3 |
Buffer | HEPES |
Specific gravity | 1.1 |
Storage | 4°C for long-term |
Structure |  |
Order number | Products | Shipment Temperature | Storage Temperature |
18411 | Glyco-DIBMA, 10x50mg | Ambient temperature | -20°C |
18412 | Glyco-DIBMA, 1g | Ambient temperature | -20°C |
18413 | Glyco-DIBMA, 10x1g | Ambient temperature | -20°C |
ask for price
Brown C.J. , Trieber C.
sciencedirect.
Overduin M., Trieber C., Prosser R.S., Picard L-P., Sheff J.G.
membranes.
Voskoboynikova, N.; Orekhov, P.; Bozdaganyan, M.; Kodde, F.; Rademacher, M.; Schowe, M.; Budke-Gieseking, A.; Brickwedde, B.; Psathaki, O.-E.; Mulkidjanian, A.Y.; Cosentino, K.; Shaitan, K.V.; Steinhoff, H.-J.
MDPI.
Voskoboynikova, N.; Margheretis E.G., Kodde F., Redemacher M., Schowe M., Budke-Gieseking A., Psathaki O-E-, Steinhoff H-J., Cosentino K.
sciencedirect.