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SMALP 140-I
The SMALP 140-I, our other SMALP products has been developed for for the solubilization of membrane proteins. SMALP 140-I is special among other SMAs as it contains a maleimide function and therefore its standard structure derives from standard SMAs. After dissolving buffer powder + SMALP 140-I in dH2O, the product is ready-to-use. SMALP 140-I has a brownish color in solution. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.
FEATURE | |
State | Lyophilized Powder, to be solved with water |
Polymer styrene-to-maleic anhydride ratio | 1.4:1 |
Molecular weight | 5,000 Da |
pH after solving | 7.5 |
Solubility | 38 % |
Divalent cationic tolerance | > 100 mM |
Color | Yellowish |
Odor | Odorless |
Freeze-thaw stable? | Yes |
Storage | 4°C for long-term |
Other noteworthy feature | Is imidized with DMAPA |
Structure |  |
Order number | Products | Shipment Temperature | Storage Temperature |
18230 | SMALP 140-I 10x50 mg | Ambient temperature | -20°C |
18231 | SMALP 140-I 1 g | Ambient temperature | -20°C |
18232 | SMALP 140-I 10 g | Ambient temperature | -20°C |
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Noh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L.
1.Univerzita Karlova Přírodovědecká fakulta.
Zhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C.,Smith Ill A.B.S, Sodroski J.G.
bioRxiv.