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Ultrasolute™ Amphipol 18

Introduction

Ultrasolute™ Amphipol 18

Ultrasolute™ Amphipol 18 from Cube Biotech is a highly purified copolymer of cyclooctyl acrylamide (COA) and acrylic acid (AA), with a molecular weight (Mw) of ~7.300. After dissolving, the copolymer is in a concentration from 1.0 to 5.0%.

Details

Ultrasolute™ Amphipol 18 from Cube Biotech is a highly purified copolymer of cyclooctyl acrylamide and acrylic acid, with a molecular weight (Mw) of ~7.300. After dissolving, the copolymer is in a concentration from 1.0 to 5.0%. Copolymers provide a hydrophobic surface facing the membrane phospholipids and a hydrophilic surface at the hydrophilic outside. This setup makes nanodiscs highly soluble in aqueous solutions and allows for the solubilization of membrane proteins in the absence of detergents. As a secondary possibility the products can be used to creat 100% artificial nanodiscs. Phospholipids such as dimyristoyl-glycero-phosphocholine (DMPC) or palmitoyl-oleoyl-phosphatidyl-choline (POPC) have been proven to work here in combination with sodium cholate. The complex of Ultrasolute™ Amphipol 18 and membrane protein can be used in many biophysical assays, such as SDS-PAGE, SEC, Western Blot, UV/Vis spectroscopy, and many chromatographic procedures. Ultrasolute™ Amphipol 18 is part of our Synthetic Nanodisc Screening Kit MAXI. Together with 16 other nanodisc polymers you can screen for the best one for your membrane protein.

Parameter

FEATURE
State	Lyophilized Powder, to be solved with water
Alternative names	CyclAPol C8-C0-50
Buffer	HEPES
pH after solving	7.5
Adsorbance	< 0.1 (280 nm, 1% solution)
COA/AA Ratio	1:1
Solubility	> 10% (H2O)
Structure

Characteristics

Order number	Products	Shipment Temperature	Storage Temperature
18321	Ultrasolute Amphipol 18, 10x50mg	Ambient temperature	-20°C
18322	Ultrasolute Amphipol 18, 1g	Ambient temperature	-20°C
18323	Ultrasolute Amphipol 18, 10x1g	Ambient temperature	-20°C

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Applications

Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers.

Marconnet A., Michon B., Prost B., Le Bon C., Giusti F., Tribet C., Zoonens M.

Biomacromolecules 2020, 21, 8, 3459–3467.

Influence of Hydrophobic Groups Attached to Amphipathic Polymers on the Solubilization of Membrane Proteins along with Their Lipids.

Marconnet A., Michon B., Prost B., Solgadi A., Le Bon C., Giusti F., Tribet C., Zoonens M.

Anal. Chem. 2022, 94, 41, 14151–14158.

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Ultrasolute™ Amphipol 18

Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers.

Influence of Hydrophobic Groups Attached to Amphipathic Polymers on the Solubilization of Membrane Proteins along with Their Lipids.